Lookup NU author(s): Dr Ricardo Martins Gouveia,
Professor Che Connon
This work is licensed under a Creative Commons Attribution 4.0 International License (CC BY 4.0).
Derivatives of fluorophore FITC (fluorescein isothiocyanate) are widely used in bioassays to label proteins and cells. An N-terminal leucine dipeptide is attached to FITC, and we show that this simple conjugate molecule is cytocompatible and is uptaken by cells (human dermal and corneal fibroblasts) in contrast to FITC itself. Co-localisation shows that FITC-LL segregates in peri-nuclear and intracellular vesicle regions. Above a critical aggregation concentration, the conjugate is shown to self-assemble into beta-sheet nanostructures comprising molecular bilayers.
Author(s): Kirkham S, Hamley IW, Smith AM, Gouveia RM, Connon CJ, Reza M, Ruokolainen J
Publication type: Article
Publication status: Published
Journal: Colloids and Surfaces B: Biointerfaces
Print publication date: 01/01/2016
Online publication date: 07/05/2015
Acceptance date: 28/04/2015
Date deposited: 17/06/2015
ISSN (print): 0927-7765
ISSN (electronic): 1873-4367
PubMed id: 25990811
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