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Characterization of the parB-like yyaA gene of Bacillus subtilis

Lookup NU author(s): Professor Jeff Errington

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Abstract

We have characterized the yyaA gene of Bacillus subtilis, located near the origin of chromosome replication (oriC). Its protein product is similar to the Spo0J protein, which belongs to the ParB family of chromosome- and plasmid-partitioning proteins. Insertional inactivation of the yyaA gene had no apparent effect on chromosome organization and partitioning during vegetative growth or sporulation. Subcellular localization of YyaA by immunofluorescence microscopy indicated that it colocalizes with the nucleoid, and gel retardation studies confirmed that YyaA binds relatively nonspecifically to DNA. Overexpression of yyaA caused a sporulation defect characterized by the formation of multiple septa within the cell. This phenotype indicates that YyaA may have a regulatory role at the onset of sporulation.


Publication metadata

Author(s): Sievers J, Raether B, Perego M, Errington J

Publication type: Article

Publication status: Published

Journal: Journal of Bacteriology

Year: 2002

Volume: 184

Issue: 4

Pages: 1102-1111

ISSN (print): 0021-9193

ISSN (electronic): 1098-5530

Publisher: American Society for Microbiology

URL: http://dx.doi.org/10.1128/jb.184.4.1102-1111.2002

DOI: 10.1128/jb.184.4.1102-1111.2002


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