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Lookup NU author(s): Dr Tomas Barta, Dr Joseph Collin, Dr Irina Neganova, Professor Lyle Armstrong, Professor Majlinda LakoORCiD
This work is licensed under a Creative Commons Attribution 4.0 International License (CC BY 4.0).
MicroRNA (miRNAs) are short non-coding RNA molecules involved in many cellular processes and shown to play a key role in somatic cell induced reprogramming. We performed an array based screening to identify candidates that are differentially expressed between dermal skin fibroblasts (DFs) and induced pluripotent stem cells (iPSCs). We focused our investigations on mir-145 and showed that this candidate is highly expressed in DFs relative to iPSCs and significantly down-regulated during reprogramming process. Inhibition of miR-145 in DFs led to the induction of “cellular plasticity” demonstrated by: i) alteration of cell morphology associated with down-regulation of mesenchymal and up-regulation of epithelial markers; ii) up-regulation of pluripotency-associated genes including SOX2, KLF4, C-MYC; iii) down-regulation of miRNA let-7b known to inhibit reprogramming and iv) increased efficiency of reprogramming to iPSCs in the presence of reprogramming factors. Together, our results indicate a direct functional link between miR-145 and molecular pathways underlying reprogramming of somatic cells to iPSCs.
Author(s): Barta T, Peskova L, Collin J, Montaner D, Neganova I, Armstrong L, Lako M
Publication type: Article
Publication status: Published
Journal: Stem Cells
Year: 2016
Volume: 34
Issue: 1
Pages: 246-251
Print publication date: 01/01/2016
Online publication date: 09/10/2015
Acceptance date: 24/08/2015
Date deposited: 23/09/2015
ISSN (print): 1066-5099
ISSN (electronic): 1549-4918
Publisher: AlphaMed Press
URL: http://dx.doi.org/10.1002/stem.2220
DOI: 10.1002/stem.2220
PubMed id: 26418476
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