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Localised controlled release of simvastatin from porous chitosan-gelatin scaffolds engrafted with simvastatin loaded PLGA-microparticles for bone tissue engineering application

Lookup NU author(s): Dr Piergiorgio Gentile

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Abstract

Localised controlled release of simvastatin from porous freeze-dried chitosan–gelatin (CH–G) scaffolds was investigated by incorporating simvastatin loaded poly-(dl-lactide-co-glycolide) acid (PLGA) microparticles (MSIMs) into the scaffolds. MSIMs at 10% w/w simvastatin loading were prepared using a single emulsion-solvent evaporation method. The MSIM optimal amount to be incorporated into the scaffolds was selected by analysing the effect of embedding increasing amounts of blank PLGA microparticles (BL-MPs) on the scaffold physical properties and on the in vitro cell viability using a clonal human osteoblastic cell line (hFOB). Increasing the BL-MP content from 0% to 33.3% w/w showed a significant decrease in swelling degree (from 1245 ± 56% to 570 ± 35%). Scaffold pore size and distribution changed significantly as a function of BL-MP loading. Compressive modulus of scaffolds increased with increasing BL-MP amount up to 16.6% w/w (23.0 ± 1.0 kPa). No significant difference in cell viability was observed with increasing BL-MP loading. Based on these results, a content of 16.6% w/w MSIM particles was incorporated successfully in CH–G scaffolds, showing a controlled localised release of simvastatin able to influence the hFOB cell proliferation and the osteoblastic differentiation after 11 days.


Publication metadata

Author(s): Gentile P, Nandagiri VK, Daly J, Chiono V, Mattu C, Tonda-Turo C, Ciardelli G, Ramtoola Z

Publication type: Article

Publication status: Published

Journal: Materials Science and Engineering C

Year: 2016

Volume: 59

Pages: 249-257

Print publication date: 01/02/2016

Online publication date: 08/10/2015

Acceptance date: 05/10/2015

ISSN (print): 0928-4931

ISSN (electronic): 1873-0191

Publisher: Elsevier

URL: http://dx.doi.org/10.1016/j.msec.2015.10.014

DOI: 10.1016/j.msec.2015.10.014


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