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Influence of Parathyroid Hormone-Loaded PLGA Nanoparticles in Porous Scaffolds for Bone Regeneration

Lookup NU author(s): Dr Piergiorgio GentileORCiD

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Abstract

Biodegradable poly(lactide-co-glycolide) (PLGA) nanoparticles, containing human parathyroid hormone (PTH (1-34)), prepared by a modified double emulsion-solvent diffusion-evaporation method, were incorporated in porous freeze-dried chitosan-gelatin (CH-G) scaffolds. The PTH-loaded nanoparticles (NPTH) were characterised in terms of morphology, size, protein loading, release kinetics and in vitro assessment of biological activity of released PTH and cytocompatibility studies against clonal human osteoblast (hFOB) cells. Structural integrity of incorporated and released PTH from nanoparticles was found to be intact by using Tris-tricine SDS-PAGE. In vitro PTH release kinetics from PLGA nanoparticles were characterised by a burst release followed by a slow release phase for 3-4 weeks. The released PTH was biologically active as evidenced by the stimulated release of cyclic AMP from hFOB cells as well as increased mineralisation studies. in vitro and cell studies demonstrated that the PTH bioactivity was maintained during the fabrication of PLGA nanoparticles and upon release. Finally, a content of 33.3% w/w NPTHs was incorporated in CH-G scaffolds, showing an intermittent release during the first 10 days and, followed by a controlled release over 28 days of observation time. The increased expression of Alkaline Phosphatase levels on hFOB cells further confirmed the activity of intermittently released PTH from scaffolds.

Publication metadata

Author(s): Gentile P, Nandagiri VK, Pabari R, Daly J, Tonda-Turo C, Ciardelli G, Ramtoola Z

Publication type: Article

Publication status: Published

Journal: International Journal of Molecular Sciences

Year: 2015

Volume: 16

Issue: 9

Pages: 20492-20510

Print publication date: 28/08/2015

Online publication date: 28/08/2015

Acceptance date: 07/08/2015

ISSN (print): 1661-6596

ISSN (electronic): 1422-0067

Publisher: MDPIAG

URL: http://www.mdpi.com/1422-0067/16/9/20492

DOI: 10.3390/ijms160920492

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