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Carbohydrate-binding module assisting glycosynthase-catalysed polymerizations

Lookup NU author(s): Professor Harry Gilbert

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Abstract

Carbohydrate-binding modules (CBMs) are found within multi-modular polysaccharide degrading enzymes [glycoside hydrolases (GHs)]. CBMs play a critical role in the recognition of plant cell-wall polysaccharides and enhance the hydrolase activity of their cognate catalytic domains by increasing enzyme substrate proximity. Mimicking their role in Nature, we, in the present study, propose that CBMs may assist in vitro glycosynthase-catalysed polymerization reactions to produce artificial polysaccharides. Glycosynthases are GHs that have been engineered to catalyse glycoside bond formation for the synthesis of oligosaccharides, glycoconjugates and glycans. The degree of polymerization (DP) of the glycans generated is limited by the solubility of the polymeric product. In the present study, we have targeted the synthesis of artificial 1,3-1,4-beta-glucans with a regular sequence using the glycosynthase (ES)-S-134 derived from a Bacillus licheniformis lichenase. We show that the addition of CBM11, which binds mixed-linked beta-glucans, either as an isolated protein or fused to the glycosynthase (ES)-S-134, has an effect on the DP of the polysaccharide products that is dependent on the rate of polymerization. The mechanism by which CBM influences the DP of the synthesized glycans is discussed.


Publication metadata

Author(s): Codera V, Gilbert HJ, Faijes M, Planas A

Publication type: Article

Publication status: Published

Journal: Biochemical Journal

Year: 2015

Volume: 470

Pages: 15-22

Print publication date: 01/08/2015

Online publication date: 11/06/2015

Acceptance date: 02/06/2015

ISSN (print): 0264-6021

ISSN (electronic): 1470-8728

Publisher: Portland Press Ltd

URL: http://dx.doi.org/10.1042/BJ20150420

DOI: 10.1042/BJ20150420


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