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Electrochemical Modification of Titanium Alloy Influences Osteoblast Morphology and Activity of Cadherin-11 and Rho-Family GTPases

Lookup NU author(s): Sarah Johnson-Lynn, Professor Sudipta Roy, Professor Andrew McCaskie, Dr Mark Birch

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Abstract

Ti alloy surfaces that ranged from rough (average Ra 1.65 mu m) to smooth (Ra 0.03 mu m) were created by electrochemical processing. Rat osteoblasts were cultured on the Ti alloy for up to 7 days and cell adhesion events (24 hours) demonstrated significant differences in cell polarity, area, mean focal adhesion area and mean number of focal adhesions per unit cell area between untreated and electrochemically polished Ti alloy. To address the mechanisms underlying phenotypic differences between cells on these surfaces, the activities of Rho-family GTPases and the localisation of cadherin-11 were investigated. Elevated RhoA activity was detected in cells on the smoother surfaces whilst on all surfaces inhibition of both RhoA and Rac1 compromised cell morphology but this was especially pronounced on the rougher surfaces. Significant differences in staining for cadherin-11 were observed in cells cultured for 7 days. On rougher surfaces, there was little evidence of cadherin-11 positive structures at the periphery of the cells however on the smoother surfaces there were extensive adherens junctions. These data illustrate that modifying implant surface structure not only influences initial cell adhesion but also has consequences for the cellular activity of intracellular mediators and the way in which cells interact with each other.


Publication metadata

Author(s): Johnson-Lynn SE, Roy S, McCaskie AW, Birch MA

Publication type: Article

Publication status: Published

Journal: Journal of Biomaterials and Tissue Engineering

Year: 2015

Volume: 5

Issue: 11

Pages: 857-863

Print publication date: 01/11/2015

Acceptance date: 01/01/1900

ISSN (print): 2157-9083

ISSN (electronic): 2157-9091

Publisher: American Scientific Publishers

URL: http://dx.doi.org/10.1166/jbt.2015.1375

DOI: 10.1166/jbt.2015.1375


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