Lookup NU author(s): Dr Thomas Penfold
This work is licensed under a Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International License (CC BY-NC-ND).
The biological influence of radiation on living matter has been studied for years; however, several questions about the detailed mechanism of radiation damage formation remain largely unanswered. Among all biomolecules exposed to radiation, DNA plays an important role because any damage to its molecular structure can affect the whole cell and may lead to chromosomal rearrangements resulting in genomic instability or cell death. To identify and characterize damage induced in the DNA sugar-phosphate backbone, in this work we performed x-ray absorption spectroscopy at the P K-edge on DNA irradiated with either UVA light or protons. By combining the experimental results with theoretical calculations, we were able to establish the types and relative ratio of lesions produced by both UVA and protons around the phosphorus atoms in DNA.
Author(s): Czapla-Masztafiak J, Szlachetko J, Milne CJ, Lipiec E, Sa J, Penfold TJ, Huthwelker T, Borca C, Abela R, Kwiatek WM
Publication type: Article
Publication status: Published
Journal: Biophysical Journal
Print publication date: 29/03/2016
Online publication date: 29/03/2016
Acceptance date: 27/01/2016
Date deposited: 01/04/2016
ISSN (print): 0006-3495
ISSN (electronic): 1542-0086
Publisher: Cell Press
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