Lookup NU author(s): Dr Birgit Koch,
Professor Natalio Krasnogor
This work is licensed under a Creative Commons Attribution 4.0 International License (CC BY 4.0).
Microfluidics may revolutionize our ability to write synthetic DNA by addressing several fundamental limitations associated with generating novel genetic constructs. Here we report the first de novo synthesis and cell-free cloning of custom DNA libraries in sub-microliter reaction droplets using programmable digital microfluidics. Specifically, we developed Programmable Order Polymerization (POP), Microfluidic Combinatorial Assembly of DNA (M-CAD) and Microfluidic In-vitro Cloning (MIC) and applied them to de novo synthesis, combinatorial assembly and cell-free cloning of genes, respectively. Proof-of-concept for these methods was demonstrated by programming an autonomous microfluidic system to construct and clone libraries of yeast ribosome binding sites and bacterial Azurine, which were then retrieved in individual droplets and validated. The ability to rapidly and robustly generate designer DNA molecules in an autonomous manner should have wide application in biological research and development.
Author(s): Ben Yehezkel T, Rival A, Raz O, Cohen R, Marx Z, Camara M, Dubern JF, Koch B, Heeb S, Krasnogor N, Delattre C, Shapiro E
Publication type: Article
Publication status: Published
Journal: Nucleic Acids Research
Print publication date: 29/02/2016
Online publication date: 19/10/2015
Acceptance date: 03/10/2015
Date deposited: 07/11/2016
ISSN (print): 0305-1048
ISSN (electronic): 1362-4962
Publisher: Oxford University Press
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