Toggle Main Menu Toggle Search

Open Access padlockePrints

Comparative mass spectrometric and immunoassay-based proteome analysis in serum of Duchenne muscular dystrophy patients

Lookup NU author(s): Dr Mattia Calissano, Professor Hanns Lochmuller, Professor Annemieke Aartsma-Rus

Downloads

Full text for this publication is not currently held within this repository. Alternative links are provided below where available.


Abstract

Purpose: Duchenne muscular dystrophy (DMD) is a severe and fatal neuromuscular disease. With the current developments on novel therapeutic strategies for DMD, the need to carefully monitor disease progression or regression upon treatment using molecular markers has become urgent.Experimental design: 2D LC protein fractionation was performed on patient serum samples, followed by LC-MS/MS-based identifications with label-free quantifications.Results: Protein signatures were compared between patients and healthy (child and adult) controls and between ambulant and nonambulant patients. Various myofibrillar proteins demonstrated differences between DMD patients and controls, likely due to leakiness and breakdown of muscle fibers. Previously reported biomarkers, such as muscle-derived titin, myosin, and carbonic anhydrase I (CA1), were verified. MS-based results were compared with ELISA for vitamin D binding protein (GC), fibulin-1 (FBLN1), gelsolin (GSN), and carbonic anhydrase 1 (CA1).Conclusions and clinical relevance: The combined results of MS-and ELISA-based quantifications indicated more studies are needed to validate this serum protein signature for DMD patients. With these data promising candidate biomarkers have been identified for a rare genetic disease using serum proteome analysis.


Publication metadata

Author(s): Oonk S, Spitali P, Hiller M, Switzar L, Dalebout H, Calissano M, Lochmuller H, Aartsma-Rus A, 't Hoen PAC, van der Burgt YEM

Publication type: Article

Publication status: Published

Journal: Proteomics - Clinical Applications

Year: 2016

Volume: 10

Issue: 3

Pages: 290-299

Print publication date: 01/03/2016

Online publication date: 08/01/2016

Acceptance date: 10/12/2015

ISSN (print): 1862-8346

ISSN (electronic): 1862-8354

Publisher: Wiley - VCH Verlag GmbH & Co. KGaA

URL: http://dx.doi.org/10.1002/prca.201500044

DOI: 10.1002/prca.201500044


Altmetrics

Altmetrics provided by Altmetric


Actions

Find at Newcastle University icon    Link to this publication


Share