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Inhibitory activity of extracts of Hebridean brown seaweeds on lipase activity

Lookup NU author(s): Dr Peter Chater, Dr Matthew Wilcox, Paul Cherry, Dr Iain Brownlee, Emeritus Professor Chris Seal, Professor Jeffrey Pearson

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This work is licensed under a Creative Commons Attribution 4.0 International License (CC BY 4.0).


Abstract

The effect of three Hebridean brown seaweeds on lipase activity was assessed using a turbidimetric lipase activity assay and an in vitro simulation of the upper digestive tract. The preparations of Ascophyllum nodosum, Fucus vesiculosus, and Pelvetia canaliculata were tested; whole seaweed homogenate, sodium carbonate extract, and ethanol extracts (pellet and supernatant were tested separately). All extracts showed significant inhibition of lipase, suggesting multiple bioactive agents, potentially including alginates, fucoidans, and polyphenols. Whole homogenate extract of F. vesiculosus was the most potent inhibitor of Lipase (IC50 = 0.119 mg mL-1), followed by ethanol supernatant (IC50 = 0.159 mg mL-1) while ethanol pellet and sodium carbonate extract showed relatively weaker inhibition (IC50 = 0.360 mg mL-1 and IC50 = 0.969 mg mL-1 respectively). For A. nodosum and P. canaliculata, strongest inhibition occurred with ethanol pellet (IC50 = 0.238 and 0.228 mg mL(-1), respectively). These inhibitory effects were validated in a model gut system. The data presented herein suggests the use of seaweed as a potential weight management tool is deserving of further investigation.


Publication metadata

Author(s): Chater PI, Wilcox M, Cherry P, Herford A, Mustar S, Wheater H, Brownlee I, Seal C, Pearson J

Publication type: Article

Publication status: Published

Journal: Journal of Applied Phycology

Year: 2016

Volume: 28

Issue: 2

Pages: 1303-1313

Print publication date: 01/04/2016

Online publication date: 26/05/2015

Acceptance date: 07/05/2015

ISSN (print): 0921-8971

ISSN (electronic): 1573-5176

Publisher: Springer

URL: http://dx.doi.org/10.1007/s10811-015-0619-0

DOI: 10.1007/s10811-015-0619-0


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