Toggle Main Menu Toggle Search

ePrints

Metabolomic and proteomic analysis of serum from preterm infants with necrotising entercolitis and late-onset sepsis

Lookup NU author(s): Dr Christopher Stewart, Dr Achim Treumann, Professor Nicholas Embleton, Dr Janet Berrington

Downloads


Licence

This work is licensed under a Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International (CC BY-NC-SA 4.0).


Abstract

BACKGROUND: Necrotising enterocolitis (NEC) and late-onset sepsis (LOS) are the leading causes of death among preterm infants in the developed world. This study aimed to explore the serum proteome and metabolome longitudinally in preterm infants with NEC or LOS, matched to controls.METHODS: Nineteen patients (10 cases, 9 controls) were included. A sample 14 d prior to and following, as well as at disease diagnosis, was included for cases. Controls had serum matched at diagnosis for corresponding case. All samples (n = 39) underwent shotgun proteomic analysis, and 37 samples also underwent metabolomics analysis using ultra performance liquid chromatography-tandem mass spectrometry.RESULTS: The proteomic and metabolomic profiles of serum were comparable between all infants. Eight proteins were associated with NEC and four proteins were associated with LOS. C-reactive protein was increased in all NEC patients at diagnosis.CONCLUSION: No single protein or metabolite was detected in all NEC or LOS cases which was absent from controls; however, several proteins were identified which were associated with disease status. The differing expression of these proteins between diseased infants potentially relates to differing pathophysiology of disease. Thus, it is unlikely a single biomarker exists for NEC and/or LOS.


Publication metadata

Author(s): Stewart CJ, Nelson A, Treumann A, Skeath T, Cummings SP, Embleton ND, Berrington JE

Publication type: Article

Publication status: Published

Journal: Pediatric Research

Year: 2016

Volume: 79

Issue: 3

Pages: 425-431

Print publication date: 01/03/2016

Online publication date: 16/11/2015

Acceptance date: 22/08/2015

ISSN (print): 0031-3998

ISSN (electronic): 1530-0447

Publisher: Nature Publishing Group

URL: http://dx.doi.org/10.1038/pr.2015.235

DOI: 10.1038/pr.2015.235


Altmetrics

Altmetrics provided by Altmetric


Actions

    Link to this publication


Share