Lookup NU author(s): Dr Jon Marles-Wright
This work is licensed under a Creative Commons Attribution 4.0 International License (CC BY 4.0).
Sortase A (SrtA) from Gram positive pathogens is an attractive target for inhibitors due to its role in the attachment of surface proteins to the cell wall. We found that the plant natural product trans-chalcone inhibits Streptococcus mutans SrtA in vitro and also inhibited S. mutans biofilm formation. Mass spectrometry revealed that the trans-chalcone forms a Michael addition adduct with the active site cysteine. The X-ray crystal structure of the SrtA H139A mutant provided new insights into substrate recognition by the sortase family. Our study suggests that chalcone flavonoids have potential as sortase-specific oral biofilm inhibitors.
Author(s): Wallock-Richards DJ, Marles-Wright J, Clarke DJ, Maitra A, Dodds M, Hanley B, Campopiano DJ
Publication type: Article
Publication status: Published
Journal: Chemical Communications
Print publication date: 04/07/2015
Online publication date: 18/05/2015
Acceptance date: 12/05/2015
Date deposited: 13/06/2016
ISSN (print): 1359-7345
ISSN (electronic): 1364-548X
Publisher: Royal Society of Chemistry
PubMed id: 26029850
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