Lookup NU author(s): Christopher Sauer,
Professor Colin Harwood,
Dr Leendert Hamoen
This work is licensed under a Creative Commons Attribution-NonCommercial 4.0 International License (CC BY-NC 4.0).
A fixed gene copy number is important for the in silico construction of engineered synthetic networks. However, the copy number of integrated genes depends on their genomic location. This gene dosage effect is rarely addressed in synthetic biology. Two studies in Escherichia colipresented conflicting data on the impact of gene dosage. Here, we investigate how genome location and gene orientation influences expression in Bacillus subtilis. An important difference with the E. coli studies is that we used an unbiased genome integration approach mediated by random transposon insertion. We found that there is a strong gene dosage effect in fast growing B. subtilis cells, which can amount to a 5-fold difference in gene expression. In contrast, gene orientation with respect to DNA replication direction does not influence gene expression. Our study shows that gene dosage should be taken into account when designing synthetic circuits in B. subtilis and presumably other bacteria.
Author(s): Sauer C, Syvertsson S, Bohorquez LC, Cruz R, Harwood CR, van Rij T, Hamoen LW
Publication type: Article
Publication status: Published
Journal: ACS Synthetic Biology
Print publication date: 16/09/2016
Online publication date: 20/05/2016
Acceptance date: 20/05/2016
Date deposited: 18/07/2016
ISSN (electronic): 2161-5063
Publisher: American Chemical Society
Altmetrics provided by Altmetric