Toggle Main Menu Toggle Search

Open Access padlockePrints

Circulating specific antibodies enhance systemic cross-priming by delivery of complexed antigen to dendritic cells in vivo

Lookup NU author(s): Dr Ingrid Verhaart

Downloads

Full text for this publication is not currently held within this repository. Alternative links are provided below where available.


Abstract

Increasing evidence suggests that antibodies can have stimulatory effects on T-cell immunity. However, the contribution of circulating antigen-specific antibodies on MHC class I cross-priming in vivo has not been conclusively established. Here, we defined the role of circulating antibodies in cross-presentation of antigen to CD8+ T cells. Micewith hapten-specific circulating antibodies, but naïve for the T-cell antigen, were infused with haptenated antigen and CD8+ T-cell induction was measured. Mice with circulating hapten-specific antibodies showed significantly enhanced cross-presentation of the injected antigen compared with mice that lacked these antibodies. The enhanced cross-presentation in mice with circulating antigen-specific antibodies was associated with improved antigen capture by APCs. Importantly, CD11c+ APCs were responsible for the enhanced and sustained cross-presentation, although CD11c+ APCs had initially captured a significant amount of the injected antigen. Thus, in vivo formation of antigen–antibody immune complexes improves MHC class I cross-presentation, and CD8+ T-cell activation, demonstrating that humoral immunity can aid the initiation of systemic cellular immunity. These findings have important implications for the understanding of the action of therapeutic antibodies against tumor-associated antigens intensively used in the clinic nowadays.


Publication metadata

Author(s): van Montfoort N, Mangsbo SM, Camps MG, van Maren WW, Verhaart IE, Waisman A, Drijfhout JW, Melief CJ, Verbeek JS, Ossendorp F

Publication type: Article

Publication status: Published

Journal: European Journal of Immunology

Year: 2012

Volume: 42

Issue: 3

Pages: 598-606

Print publication date: 01/03/2012

Online publication date: 11/04/2012

ISSN (print): 0014-2980

ISSN (electronic): 1521-4141

Publisher: Wiley - VCH Verlag GmbH & Co. KGaA

URL: http://dx.doi.org/10.1002/eji.201141613

DOI: 10.1002/eji.201141613

PubMed id: 22488363


Altmetrics

Altmetrics provided by Altmetric


Actions

Find at Newcastle University icon    Link to this publication


Share