Lookup NU author(s): Dr Piergiorgio Gentile
This work is licensed under a Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International License (CC BY-NC-ND).
The incorporation of a bioactive inorganic phase in polymeric scaffolds is a good strategy for the improvement of the bioactivity and the mechanical properties, which represent crucial features in the field of bone tissue engineering. In this study, spray-dried mesoporous bioactive glass particles (SD-MBG), belonging to the binary system of SiO2-CaO (80:20 mol%), were used to prepare composite scaffolds by freeze-drying technique, using a silk fibroin matrix. The physico-chemical and biological properties of the scaffolds were extensively studied. The scaffolds showed a highly interconnected porosity with a mean pore size in the range of 150 gm for both pure silk and silk/SD-MBG scaffolds. The elastic moduli of the silk and silk/SD-MBG scaffolds were 1.1 +/- 0.2 MPa and 6.9 +/- 1.0 MPa and compressive strength were 0.5 +/- 0.05 MPa and 0.9 +/- 0.2 MPa, respectively, showing a noticeable increase of the mechanical properties of the composite scaffolds compared to the silk ones. The contact angle value decreased from 105.3 degrees to 71.2 degrees with the incorporation of SD-MBG particles. Moreover, the SD-MBG incorporation countered the lack of bioactivity of the silk scaffolds inducing the precipitation of hydroxyapatite layer on their surface already after 1 day of incubation in simulated body fluid. The composite scaffolds showed good biocompatibility and a good alkaline phosphatase activity toward human mesenchymal stromal cells, showing the ability for their use as three-dimensional constructs for bone tissue engineering. (C) 2016 Elsevier Ltd and Techna Group S.r.l. All rights reserved.
Author(s): Chandrasekaran A, Novajra G, Carmagnola I, Gentile P, Fiorilli S, Miola M, Boregowda M, Dakshanamoorthy A, Ciardelli G, Vitale-Brovarone C
Publication type: Article
Publication status: Published
Journal: Ceramics International
Print publication date: 01/09/2016
Online publication date: 27/05/2016
Acceptance date: 06/05/2016
ISSN (print): 0272-8842
ISSN (electronic): 1873-3956
Publisher: Elsevier Science Ltd.
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