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Synthesis of β-1,4-Linked Galactan Side-Chains of Rhamnogalacturonan I

Lookup NU author(s): Professor William Willats

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Abstract

The synthesis of linear- and (1 -> 6)-branched beta-(1 -> 4)-D-galactans, side-chains of the pectic polysaccharide rhamnogalacturonan I is described. The strategy relies on iterative couplings of n-pentenyl disaccharides followed by a late stage glycosylation of a common hexasaccharide core. Reaction with a covalent linker and immobilization on N-hydroxysuccinimide (NHS)-modified glass surfaces allows the generation of carbohydrate microarrays. The glycan arrays enable the study of protein-carbohydrate interactions in a high-throughput fashion, demonstrated herein with binding studies of mAbs and a CBM.


Publication metadata

Author(s): Andersen MCF, Kracun SK, Rydahl MG, Willats WGT, Clausen MH

Publication type: Article

Publication status: Published

Journal: Chemistry: A European Journal

Year: 2016

Volume: 22

Issue: 33

Pages: 11543-11548

Print publication date: 01/08/2016

Online publication date: 12/07/2016

Acceptance date: 15/06/2016

ISSN (print): 0947-6539

ISSN (electronic): 1521-3765

Publisher: Wiley - V C H Verlag GmbH & Co. KGaA

URL: http://dx.doi.org/10.1002/chem.201602197

DOI: 10.1002/chem.201602197


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