Lookup NU author(s): Professor William Willats
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The synthesis of linear- and (1 -> 6)-branched beta-(1 -> 4)-D-galactans, side-chains of the pectic polysaccharide rhamnogalacturonan I is described. The strategy relies on iterative couplings of n-pentenyl disaccharides followed by a late stage glycosylation of a common hexasaccharide core. Reaction with a covalent linker and immobilization on N-hydroxysuccinimide (NHS)-modified glass surfaces allows the generation of carbohydrate microarrays. The glycan arrays enable the study of protein-carbohydrate interactions in a high-throughput fashion, demonstrated herein with binding studies of mAbs and a CBM.
Author(s): Andersen MCF, Kracun SK, Rydahl MG, Willats WGT, Clausen MH
Publication type: Article
Publication status: Published
Journal: Chemistry: A European Journal
Print publication date: 01/08/2016
Online publication date: 12/07/2016
Acceptance date: 15/06/2016
ISSN (print): 0947-6539
ISSN (electronic): 1521-3765
Publisher: Wiley - V C H Verlag GmbH & Co. KGaA
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