Lookup NU author(s): Dr Ana Ferreira-Duarte,
Dr Piergiorgio Gentile,
Dr Ria Toumpaniari,
Dr Mark Birch
This work is licensed under a Creative Commons Attribution-NonCommercial 4.0 International License (CC BY-NC 4.0).
Bone cell interaction with extracellular matrix (ECM) microenvironment is of critical importance when engineering surface interfaces for bone regeneration. In this work layer-by-layer films of type I collagen (coll), the major constituent of bone ECM, and heparin (hep), a glycosaminoglycan, were assembled on poly(L-lactide acid) (PLLA) substrates to evaluate the impact of the biomacromolecular coating on cell activity. The surface modification of PLLA demonstrated that the hep/coll multi-layer is stable after 10 bilayers (confirmed by contact angle, infrared spectroscopy and morphological analysis). This simple approach provided novel information on the effect of heparin on type I collagen hierarchical organisation and subsequent cell response of osteoblast-like (MC3T3-E1) and human bone marrow-derived mesenchymal stem cells (hMSCs). Interestingly, the number of deposited heparin layers (1 or 10) appeared to play an important role in the self-assembly of collagen into fibrils, stabilising the fibrous collagen layer, and potentially impacting on hMSCs activity.
Author(s): Ferreira AM, Gentile P, Toumpaniari S, Ciardelli G, Birch MA
Publication type: Article
Publication status: Published
Journal: ACS Applied Materials & Interfaces
Online publication date: 20/10/2016
Acceptance date: 18/10/2016
ISSN (print): 1944-8244
ISSN (electronic): 1944-8252
Publisher: American Chemical Society
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