Lookup NU author(s): Dr Pamela Gamba,
Dr Leendert Hamoen,
Dr Richard Daniel
This work is licensed under a Creative Commons Attribution 4.0 International License (CC BY 4.0).
Five essential proteins are known to assemble at the division site of Bacillus subtilis. However, the recruitment of the FtsW homolog is still unclear. Here, we take advantage of spore germination to facilitate the depletion of essential proteins and to study the divisome assembly in the absence of previous division events. We show that, unlike what has been shown for the Escherichia coli divisome, the assembly of FtsW is interdependent with the localization of PBP 2B and FtsL, which are key components of the membrane bound division complex. Interestingly, the Z-ring appeared to disassemble upon prolonged depletion of late division proteins. Nevertheless, we could restore Z-ring formation and constriction by re-inducing FtsW, which suggests that the stability of the Z-ring is stimulated by the assembly of a functional division complex.
Author(s): Gamba P, Hamoen LW, Daniel RA
Publication type: Article
Publication status: Published
Journal: Frontiers in Microbiology
Online publication date: 15/11/2016
Acceptance date: 27/10/2016
Date deposited: 19/01/2017
ISSN (print): 1664-302X
Publisher: Frontiers Research Foundation
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