Lookup NU author(s): Professor William Willats
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Plant cell wall glycans are important polymers that are crucial to plant development and serve as an important source of sustainable biomass. The study of polysaccharides in the plant cell wall relies heavily on monoclonal antibodies (mAbs) for localization and visualization of glycans, using e.g. immunofluorescent microscopy. Here, we describe the detailed epitope mapping of the mAb LM5 that is shown to bind to a minimum of three sugar residues at the non-reducing end of linear beta-1,4-linked galactan. The study uses de novo synthetic analogues of galactans combined with carbohydrate microarray and competitive inhibition ELISA for analysis of antibody-carbohydrate interactions.
Author(s): Andersen MCF, Boos I, Marcus SE, Kračun SK, Rydahl MG, Willats WGT, Knox JP, Clausen MH
Publication type: Article
Publication status: Published
Journal: Carbohydrate Research
Print publication date: 21/12/2016
Online publication date: 30/10/2016
Acceptance date: 27/10/2016
ISSN (print): 0008-6215
ISSN (electronic): 1873-426X
Publisher: Pergamon Press
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