Lookup NU author(s): Dr Satomi Miwa,
Dr Rafal Czapiewski,
Professor Thomas von Zglinicki,
Dr Gabriele Saretzki
This work is licensed under a Creative Commons Attribution 4.0 International License (CC BY 4.0).
Telomerase in its canonical function maintains telomeres in dividing cells. In addition, the telomerase protein TERT has non-telomeric functions such as shuttling to mitochondria resulting in a decreased oxidative stress, DNA damage and apoptosis. TERT protein persists in adult neurons and can co-localise to mitochondria under various stress conditions. We show here that TERT expression decreased in mouse brain during aging while release of reactive oxygen species (ROS) from the mitochondrial electron transport chain increased. Dietary restriction (DR) caused accumulation of TERT protein in mouse brain mitochondria correlating to decreased ROS release and improved learning and spatial short-term memory. Decreased mTOR signalling is a mediator of DR. Accordingly, feeding mice with rapamycin increased brain mitochondrial TERT and reduced ROS release. Importantly, the beneficial effects of rapamycin on mitochondrial function were absent in brains and fibroblasts from first generation TERT -/-mice, and when TERT shuttling was inhibited by the Src kinase inhibitor bosutinib. Taken together, our data suggests that the mTOR signalling pathway impinges on the mitochondrial localisation of TERT protein, which might in turn contribute to the protection of the brain by DR or rapamycin against age-associated mitochondrial ROS increase and cognitive decline.
Author(s): Miwa S, Czapiewski R, Wan TF, Bell A, Hill KN, von Zglinicki T, Saretzki G
Publication type: Article
Publication status: Published
Online publication date: 22/10/2016
Acceptance date: 02/10/2016
Date deposited: 10/05/2017
ISSN (print): 1945-4589
Publisher: Impact Journal LLC
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