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In vitro and in vivo evaluation of two carrageenan-based formulations to prevent HPV acquisition

Lookup NU author(s): Dr Brian Ford

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This work is licensed under a Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International License (CC BY-NC-ND).


Abstract

Commercial vaccines against human papillomavirus (HPV) have low uptake due to parental autonomy, dosing regimen, cost, and cold chain storage requirements. Carrageenan (CG)-based formulations prevent HPV infection in vitro and in vivo but data are needed on the durability of anti-HPV activity and the effect of seminal plasma (SP). The Population Council's PC-515 gel and the lubricant Divine 9 were tested for their physicochemical properties and anti-HPV activity against HPV16, 18, and 45 pseudoviruses (PsVs). Anti-PsV activity was estimated using the luciferase assay in HeLa cells and the HPV PsV luciferase mouse model. Formulations were applied intravaginally either 2 h pre/2 h post (-2 h/+2 h) or 24 h pre (-24 h) relative to challenge with HPV16 or 45 PsV in PBS or SP/PBS. Both formulations showed broad-spectrum anti-HPV activity in vitro (IC50: 1-20 ng/ml), significantly decreasing HPV PsV infection in the mouse model (-2 h/+2 h, p <0.0001). PC-515 protected better than Divine 9 in the 24 h dosing regimen (p < 0.0001) and comparable to Divine 9 in the 2 h/+2 h regimen (p = 0.9841). PC-515 retained full activity in the murine model when PsV solutions contained human SP. The durable, potential broad-spectrum anti-HPV activity of CG formulations in the presence of SP supports their further development to prevent HPV acquisition.


Publication metadata

Author(s): Rodriguez A, Kleinbeck K, Mizenina O, Kizima L, Levendosky K, Jean-Pierre N, Villegas G, Ford BE, Cooney ML, Teleshova N, Robbiani M, Herold BC, Zydowsky T, Romero JAF

Publication type: Article

Publication status: Published

Journal: Antiviral Research

Year: 2014

Volume: 108

Pages: 88-93

Print publication date: 01/08/2014

Online publication date: 05/06/2014

Acceptance date: 19/05/2014

Date deposited: 04/04/2018

ISSN (print): 0166-3542

ISSN (electronic): 1872-9096

Publisher: Elsevier

URL: https://doi.org/10.1016/j.antiviral.2014.05.018

DOI: 10.1016/j.antiviral.2014.05.018

PubMed id: 24909570


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