Toggle Main Menu Toggle Search

ePrints

Oral administration of amphotericin B nanoparticles: antifungal activity, bioavailability and toxicity in rats

Lookup NU author(s): Dr Bushra alQuadeib, Professor Lidija Siller, Professor Matthew Wright, Dr Ben Horrocks

Downloads


Licence

This work is licensed under a Creative Commons Attribution 4.0 International License (CC BY 4.0).


Abstract

Amphotericin B (AMB) is used most commonly in severe systemic life-threatening fungal infections. There is currently an unmet need for an efficacious (AMB) formulation amenable to oral administration with better bioavailability and lower nephrotoxicity. Novel PEGylated polylactic-polyglycolic acid copolymer (PLGA-PEG) nanoparticles (NPs) formulations of AMB were therefore studied for their ability to kill Candida albicans (C. albicans). The antifungal activity of AMB formulations was assessed in C. albicans. Its bioavalability was investigated in nine groups of rats (n = 6). Toxicity was examined by an in vitro blood hemolysis assay, and in vivo nephrotoxicity after single and multiple dosing for a week by blood urea nitrogen (BUN) and plasma creatinine (PCr) measurements. The MIC of AMB loaded to PLGA-PEG NPs against C. albicans was reduced two to threefold compared with free AMB. Novel oral AMB delivery loaded to PLGA-PEG NPs was markedly systemically available compared to Fungizone® in rats. The addition of 2% of GA to the AMB formulation significantly (p < 0.05) improved the bioavailability from 1.5 to 10.5% and the relative bioavailability was > 790% that of Fungizone®. The novel AMB formulations showed minimal toxicity and better efficacy compared to Fungizone®. No nephrotoxicity in rats was detected after a week of multiple dosing of AMB NPs based on BUN and PCr, which remained at normal levels. An oral delivery system of AMB-loaded to PLGA-PEG NPs with better efficacy and minimal toxicity was formulated. The addition of glycyrrhizic acid (GA) to AMB NPs formulation resulted in a significant oral absorption and improved bioavailability in rats.


Publication metadata

Author(s): Radwan MA, AlQuadeib BT, Siller L, Wright MC, Horrocks B

Publication type: Article

Publication status: Published

Journal: Drug Delivery

Year: 2017

Volume: 24

Issue: 1

Pages: 40-50

Online publication date: 03/02/2017

Acceptance date: 22/08/2016

ISSN (print): 1071-7544

ISSN (electronic): 1521-0464

Publisher: Taylor & Francis Inc.

URL: http://dx.doi.org/10.1080/10717544.2016.1228715

DOI: 10.1080/10717544.2016.1228715

Notes: This articles is published with Open access http://www.tandfonline.com/doi/full/10.1080/10717544.2016.1228715


Altmetrics

Altmetrics provided by Altmetric


Actions

    Link to this publication


Share