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Lipopolysaccharide from Escherichia coli prevents indomethacin-induced gastric damage in rats: role of non-protein sulfhydryl groups and leukocyte adherence.

Lookup NU author(s): Dr Henrique De Paula Lemos

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Abstract

OBJECTIVE AND DESIGN:To investigate the role of non-protein sulfhydryl groups (NP-SH) and leukocyte adhesion in the protective effect of lipopolysaccharide (LPS) from Escherichia coli against indomethacin-induced gastropathy.MATERIALS OR SUBJECTS:Male Wistar rats were divided into four groups: saline, LPS, saline + indomethacin and LPS + indomethacin, with six rats in each group.TREATMENT:Rats were pretreated with LPS (300 microg/kg, by intravenous) or saline. After 6 h, indomethacin was administered (20 mg/kg, by gavage).METHODS:Three hours after treatments, rats were killed. Macroscopic gastric damage, gastric NP-SH concentration, myeloperoxidase (MPO) activity and mesenteric leukocyte adhesion (intravital microscopy) were assessed. Statistical analysis was performed using one-way analysis of variance followed by the Newman-Keuls test. Statistical significance was set at P < 0.05.RESULTS:LPS reduced the gastric damage, gastric MPO activity and increased gastric NP-SH concentration in indomethacin-induced gastropathy. LPS alone increased gastric NP-SH when compared to saline. Indomethacin increased leukocyte adhesion when compared to the saline, and LPS reduced indomethacin-induced leukocyte adhesion. In addition, LPS alone did not change leukocyte adhesion, when compared to the saline.CONCLUSION:LPS protective effect against indomethacin-induced gastropathy is mediated by an increase in the NP-SH and a decrease in leukocyte-endothelial adhesion.


Publication metadata

Author(s): Gomes AS, Lemos HP, Medeiros JV, Cunha FQ, Souza MH

Publication type: Article

Publication status: Published

Journal: Inflammation Research

Year: 2009

Volume: 58

Issue: 10

Pages: 717-723

Print publication date: 01/10/2009

Online publication date: 29/04/2009

ISSN (print): 1023-3830

ISSN (electronic): 1420-908X

Publisher: Springer

URL: http://doi.org/10.1007/s00011-009-0040-8

DOI: 10.1007/s00011-009-0040-8

PubMed id: 19404581


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