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Lookup NU author(s): Dr Olivier GovaereORCiD
This work is licensed under a Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International License (CC BY-NC-ND).
PURPOSE. Fuchs' endothelial corneal dystrophy (FECD) is a degenerative eye disorder affecting 4% of Americans older than 40. It is the leading indication for corneal endothelial (CE) transplantation for which there is a global donor shortage. This study aimed to gain further insight into the pathophysiology of FECD and identify targets for nonsurgical therapy.METHODS. CE from patients with late-onset FECD was compared with that of normal controls using microarray expression analysis (n = 4 FECD, n = 4 normal), reverse transcriptase quantitative PCR (n = 9 FECD, n = 8 normal), and immunohistology (n = 55 FECD, n = 15 normal).RESULTS. This led to the identification of circulating fibrocytes and their dendritic derivatives in all examined CE samples with FECD (in all clinical stages of symptomatic FECD and independent of prior cataract surgery). These cells were not present in normal CE. In this study we characterize their morphology, protein expression profile, number, and localization within the CE layer of patients with FECD.CONCLUSIONS. Circulating fibrocytes and their dendritic derivatives are a new aspect of FECD that deserves further investigation. Because they are known to cause fibrosis in a variety of organs, they may play a similar role in FECD and might be a valuable target for nonsurgical therapy.
Author(s): De Roo AK, Wouters J, Govaere O, Foets B, van den Oord JJ
Publication type: Article
Publication status: Published
Journal: Investigative Ophthalmology & Visual Science
Year: 2017
Volume: 58
Issue: 1
Pages: 670-681
Print publication date: 01/01/2017
Acceptance date: 19/12/2016
Date deposited: 30/03/2017
ISSN (print): 0146-0404
ISSN (electronic): 1552-5783
Publisher: Association for Research in Vision and Ophthalmology
URL: https://doi.org/10.1167/iovs.16-20880
DOI: 10.1167/iovs.16-20880
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