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Mechanism of inhibition by hydrogen sulfide of native and recombinant BK_Ca channels

Lookup NU author(s): Dr Seva TelezhkinORCiD

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Abstract

Recent evidence suggests that H(2)S contributes to activation of the carotid body by hypoxia by inhibiting K(+) channels. Here, we determine both the molecular identity of the K(+) channel target within the carotid body and the biophysical characteristics of the H(2)S-evoked inhibition by analyzing native rat and human recombinant BK(Ca) channel activity in voltage-clamped, inside-out membrane patches. Rat glomus cells express the enzymes necessary for the endogenous generation of H(2)S, cystathionine-beta-synthase and cystathionine-gamma-lyase. H(2)S inhibits native carotid body and human recombinant BK(Ca) channels with IC(50) values of around 275 microM. Inhibition by H(2)S is rapid and reversible, works by a mechanism which is distinct from that suggested for CO gas regulation of this channel and does not involve an interaction with either the "Ca bowl" or residues distal to this Ca(2+)-sensing domain. These data show that BK(Ca) is a K(+) channel target of H(2)S, and suggest a mechanism to explain the H(2)S-dependent component of O(2) sensing in the carotid body.

Publication metadata

Author(s): Telezhkin V, Brazier SP, Cayzac S, Wilkinson WJ, Riccardi D, Kemp PJ

Publication type: Article

Publication status: Published

Journal: Respiratory Physiology & Neurobiology

Year: 2010

Volume: 172

Issue: 3

Pages: 169-178

Print publication date: 31/07/2010

Online publication date: 16/05/2010

Acceptance date: 10/05/2010

ISSN (print): 1569-9048

ISSN (electronic): 1878-1519

Publisher: Elsevier BV

URL: https://doi.org/10.1016/j.resp.2010.05.016

DOI: 10.1016/j.resp.2010.05.016

PubMed id: 20576528

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