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Lookup NU author(s): Dr Seva TelezhkinORCiD
This work is licensed under a Creative Commons Attribution 4.0 International License (CC BY 4.0).
Skin keratinocytes fulfil important signalling and protective functions. Immunocytochemical experiments revealed the unexpected presence of immunoreactivity for the M-type potassium channel subunit Kv7.2 in the keratinocyte layer of intact rat paw skin and in keratinocytes isolated from the skin of 1-day-old rats and cultured in vitro for 3-10 days. Application of the M-channel enhancer retigabine (3-10 μM) to isolated cultured rat keratinocytes: (a) increased outward membrane currents recorded under voltage clamp, (b) produced ~3 mV hyperpolarization at rest, (c) enhanced ~3-fold the release of ATP induced by the TRPV3 agonist carvacrol (1 mM) and (d) increased the amplitude of the carvacrol-induced intracellular Ca(2+) transient measured with Fura-2. The effect of retigabine on ATP release was prevented by the M-channel blocking agent XE991. We conclude that rat skin keratinocytes possess M-channels that, when activated, can modify their physiological properties, with potential significance for their sensory and other biological functions.
Author(s): Reilly JM, Telezhkin V, Passmore GM, Marsh SJ, Brown DA
Publication type: Article
Publication status: Published
Journal: Pflügers Archiv - European Journal of Physiology
Year: 2013
Volume: 465
Issue: 9
Pages: 1371-1381
Print publication date: 01/09/2013
Online publication date: 17/04/2013
Date deposited: 09/03/2017
ISSN (print): 0031-6768
Publisher: Springer
URL: http://dx.doi.org/10.1007/s00424-013-1276-2
DOI: 10.1007/s00424-013-1276-2
PubMed id: 23592175
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