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Gene Silencing by RNAi in Mammalian Cells

Lookup NU author(s): Dr Frida Ponthan, Professor Olaf Heidenreich, Dr Kelly Coffey

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Abstract

© 2015 by John Wiley & Sons, Inc.This unit provides information how to use short interfering RNA (siRNA) for sequence-specific gene silencing in mammalian cells. Several methods for siRNA generation and optimization, as well as recommendations for cell transfection and transduction, are presented.

Publication metadata

Author(s): Ponthan F, Yusoff NM, Soria NM, Heidenreich O, Coffey K

Publication type: Article

Publication status: Published

Journal: Current Protocols in Molecular Biology

Year: 2015

Volume: 2015

Pages: 26.2.1-26.2.17

Online publication date: 01/07/2015

Acceptance date: 01/01/1900

ISSN (print): 1934-3639

ISSN (electronic): 1934-3647

Publisher: Blackwell Publishing Inc.

URL: http://doi.org/ 10.1002/0471142727.mb2602s111

DOI: 10.1002/0471142727.mb2602s111

PubMed id: 26131850

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