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Green fluorescent protein as a reporter for the spatial and temporal expression of actIII in Streptomyces coelicolor

Lookup NU author(s): Professor Jeff Errington

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This work is licensed under a Creative Commons Attribution 4.0 International License (CC BY 4.0).


Abstract

© 2017 The Author(s) Polyketides constitute a large group of structurally diverse natural products with useful biological activities. Insights into their biosynthetic mechanisms are crucial for developing new structures. One of the most studied model polyketide is the blue-pigmented antibiotic actinorhodin, produced by Streptomyces coelicolor. This aromatic polyketide is synthesized by minimal type II polyketide synthases and tailoring enzymes. The ActIII actinorhodin ketoreductase is a key tailoring enzyme in actinorhodin biosynthesis. Previous papers have reported contradictory findings for localization of the protein in the cytoplasmic fraction or associated with the cell wall. We have now used green fluorescent protein as a reporter to analyse the spatial and temporal expression of actIII (SCO5086) in S. coelicolor under actinorhodin producing and non-producing conditions. We provide evidence in support of ActIII being a cytosolic protein, with limited if any association with the membrane or cell wall.


Publication metadata

Author(s): Santos-Beneit F, Errington J

Publication type: Article

Publication status: Published

Journal: Archives of Microbiology

Year: 2017

Volume: 199

Issue: 6

Pages: 875-880

Print publication date: 01/08/2017

Online publication date: 22/03/2017

Acceptance date: 07/03/2017

Date deposited: 03/05/2017

ISSN (print): 0302-8933

ISSN (electronic): 1432-072X

Publisher: Springer Verlag

URL: https://doi.org/10.1007/s00203-017-1358-1

DOI: 10.1007/s00203-017-1358-1


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