Lookup NU author(s): Dr Merry Gunawan,
Professor Muzlifah Haniffa
This is the authors' accepted manuscript of an article that has been published in its final definitive form by American Association for the Advancement of Science, 2017.
For re-use rights please refer to the publisher's terms and conditions.
© 2017 American Association for the Advancement of Science. Dendritic cells (DC) are professional antigen-presenting cells that orchestrate immune responses. The human DC population comprises two main functionally specialized lineages, whose origins and differentiation pathways remain incompletely defined. Here, we combine two high-dimensional technologies—single-cell mRNA sequencing and cytometry by time-of-flight (CyTOF), to identify human blood CD123+CD33+CD45RA+ DC precursors (pre-DC). Pre-DC share surface markers with plasmacytoid DC (pDC) but have distinct functional properties that were previously attributed to pDC. Tracing the differentiation of DC from the bone marrow to the peripheral blood revealed that the pre-DC compartment contains distinct lineage-committed subpopulations, including one early uncommitted CD123high pre-DC subset and two CD45RA+CD123low lineage-committed subsets exhibiting functional differences. The discovery of multiple committed pre-DC populations opens promising new avenues for the therapeutic exploitation of DC subset-specific targeting.
Author(s): See P, Dutertre C-A, Chen J, Gunther P, McGovern N, Irac SE, Gunawan M, Beyer M, Handler K, Duan K, Sumatoh HRB, Ruffin N, Jouve M, Gea-Mallorqui E, Hennekam RCM, Lim T, Yip CC, Wen M, Malleret B, Low I, Shadan NB, Fen CFS, Tay A, Lum J, Zolezzi F, Larbi A, Poidinger M, Chan JKY, Chen Q, Renia L, Haniffa M, Benaroch P, Schlitzer A, Schultze JL, Newell EW, Ginhoux F
Publication type: Article
Publication status: Published
Print publication date: 19/05/2017
Online publication date: 04/05/2017
Acceptance date: 25/04/2017
ISSN (print): 0036-8075
ISSN (electronic): 1095-9203
Publisher: American Association for the Advancement of Science
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