Lookup NU author(s): Professor Phillip Wright
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© 2017, Springer Science+Business Media LLC. The production of N-linked recombinant glycoproteins is possible in a variety of biotechnology host cells, and more recently in the bacterial workhorse, Escherichia coli. This methods chapter will outline the components and procedures needed to produce N-linked glycoproteins in E. coli, utilizing Campylobacter jejuni glycosylation machinery, although other related genes can be used with minimal tweaks to this methodology. To ensure a successful outcome, various methods will be highlighted that can confirm glycoprotein production to a high degree of confidence, including the gold standard of mass spectrometry analysis.
Author(s): Strutton B, Jaffe SRP, Pandhal J, Wright PC
Publication type: Book Chapter
Publication status: Published
Book Title: Methods in Molecular Biology
Online publication date: 04/05/2017
Acceptance date: 02/04/2016
Series Title: Methods in Molecular Biology
Publisher: Humana Press Inc.
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