Generation of recombinant <em>N-</em>linked glycoproteins in <em>E. coli</em>

Strutton, B; Jaffe, SRP; Pandhal, J; Wright, PC

doi:10.1007/978-1-4939-6887-9_15

Generation of recombinant N-linked glycoproteins in E. coli

Lookup NU author(s): Professor Phillip Wright ORCiD

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Abstract

© 2017, Springer Science+Business Media LLC. The production of N-linked recombinant glycoproteins is possible in a variety of biotechnology host cells, and more recently in the bacterial workhorse, Escherichia coli. This methods chapter will outline the components and procedures needed to produce N-linked glycoproteins in E. coli, utilizing Campylobacter jejuni glycosylation machinery, although other related genes can be used with minimal tweaks to this methodology. To ensure a successful outcome, various methods will be highlighted that can confirm glycoprotein production to a high degree of confidence, including the gold standard of mass spectrometry analysis.