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Detection and transmission of Carrot torrado virus, a novel putative member of the Torradovirus genus

Lookup NU author(s): Professor Neil Boonham

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This work is licensed under a Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International License (CC BY-NC-ND).


Abstract

© 2016 Elsevier B.V. A new Torradovirus tentatively named Carrot torrado virus (CaTV) was an incidental finding following a next generation sequencing study investigating internal vascular necrosis in carrot. The closest related viruses are Lettuce necrotic leaf curl virus (LNLCV) found in the Netherlands in 2011 and Motherwort yellow mottle virus (MYMoV) found in Korea in 2014. Primers for reverse transcriptase-PCR (RT-PCR) and RT-qPCR were designed with the aim of testing for the presence of virus in plant samples collected from the field. Both methods successfully amplified the target from infected samples but not from healthy control samples. The specificity of the CaTV assay was also checked against other known carrot viruses and no cross-reaction was seen. A comparative study between methods showed RT-qPCR was the most reliable method, giving positive results in samples where RT-PCR fails. Evaluation of the Ct values following RT-qPCR and a direct comparison demonstrated this was due to improved sensitivity. The previous published Torradovirus genus specific RT-PCR primers were tested and shown to detect CaTV. Also, virus transmission experiments carried out suggest that unlike other species of the same genus, Carrot torrado virus could be aphid-transmitted.


Publication metadata

Author(s): Rozado-Aguirre Z, Adams I, Collins L, Fox A, Dickinson M, Boonham N

Publication type: Article

Publication status: Published

Journal: Journal of Virological Methods

Year: 2016

Volume: 235

Pages: 119-124

Print publication date: 01/09/2016

Online publication date: 01/06/2016

Acceptance date: 29/05/2016

ISSN (print): 0166-0934

ISSN (electronic): 1879-0984

Publisher: Elsevier

URL: https://doi.org/10.1016/j.jviromet.2016.05.018

DOI: 10.1016/j.jviromet.2016.05.018


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