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RNA2 of Soil-borne cereal mosaic virus is detectable in plants of winter wheat grown from infected seeds

Lookup NU author(s): Professor Giles Budge, Professor Neil Boonham

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Abstract

Soil-borne cereal mosaic virus (SBCMV) causes a severe disease in susceptible cultivars of winter wheat. The virus is vectored by the soil-borne protist Polymyxa graminis. Experiments were conducted to investigate whether SBCMV RNA2 could persist in seed from SBCMV-infected susceptible cultivars of winter wheat. Over 7,000 seedlings were generated from seed collected from two cultivars of SBCMV-infected winter wheat. Seedlings were grown in a glasshouse compartment and batch tested for the presence of SBCMV using real-time RT-PCR. The majority of batches tested positive for SBCMV, indicating an RNA2 transmission rate of 1.8-9.4% in wheat. The presence of the virus was confirmed by amplifying and sequencing a larger (400 bp) fragment of viral RNA2 in a sub-set of the seedlings testing positive by real-time RT-PCR. Root extracts from this sub-set tested negative for P. graminis using real-time PCR. The implications for disease epidemiology of this virus are discussed. © 2007 KNPV.


Publication metadata

Author(s): Budge GE, Loram J, Donovan G, Boonham N

Publication type: Article

Publication status: Published

Journal: European Journal of Plant Pathology

Year: 2008

Volume: 120

Issue: 1

Pages: 97-102

Print publication date: 01/01/2008

Online publication date: 01/11/2007

ISSN (print): 0929-1873

ISSN (electronic): 1573-8469

URL: https://doi.org/10.1007/s10658-007-9194-9

DOI: 10.1007/s10658-007-9194-9


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