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Selenomethionine Alleviates AFB1-Induced Damage in Primary Chicken Hepatocytes by Inhibiting CYP450 1A5 Expression via Upregulated SelW Expression

Lookup NU author(s): Dr Viktor Korolchuk

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Abstract

This study aims to evaluate the protective effects of selenomethionine (SeMet) on aflatoxin B1 (AFB1)-induced hepatotoxicity in primary chicken hepatocytes. Cell viability and lactic dehydrogenase activity assays revealed the dose dependence of AFB1 toxicity to chicken hepatocytes. AFB1 concentrations of >0.05 μg/mL significantly reduced glutathione and total superoxide dismutase levels and increased the malondialdehyde concentration and cytochrome P450 enzyme 1A5 (CYP450 1A5) mRNA levels (P < 0.05). AFB1, however, did not affect CYP450 3A37 mRNA levels. Supplementation with 2 μM SeMet protected against AFB1-induced changes and significantly increased selenoprotein W (SelW) mRNA levels (P < 0.05). Additionally, SelW knockdown attenuated the protective effect of SeMet on AFB1-induced damage and significantly increased the level of CYP450 1A5 expression (P < 0.05). Therefore, SeMet alleviates AFB1-induced damage in primary chicken hepatocytes by improving SelW expression, thus inhibiting CYP450 1A5 expression.


Publication metadata

Author(s): Chen X, Che C, Korolchuk VI, Gan F, Pan C, Huang K

Publication type: Article

Publication status: Published

Journal: Journal of Agricultural and Food Chemistry

Year: 2017

Volume: 65

Issue: 12

Pages: 2495-2502

Print publication date: 29/03/2017

Online publication date: 13/03/2017

Acceptance date: 12/03/2017

ISSN (print): 0021-8561

ISSN (electronic): 1520-5118

Publisher: American Chemical Society

URL: https://doi.org/10.1021/acs.jafc.6b05308

DOI: 10.1021/acs.jafc.6b05308

PubMed id: 28285519


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