Browse by author
Lookup NU author(s): Dr Viktor Korolchuk
Full text for this publication is not currently held within this repository. Alternative links are provided below where available.
This study aims to evaluate the protective effects of selenomethionine (SeMet) on aflatoxin B1 (AFB1)-induced hepatotoxicity in primary chicken hepatocytes. Cell viability and lactic dehydrogenase activity assays revealed the dose dependence of AFB1 toxicity to chicken hepatocytes. AFB1 concentrations of >0.05 μg/mL significantly reduced glutathione and total superoxide dismutase levels and increased the malondialdehyde concentration and cytochrome P450 enzyme 1A5 (CYP450 1A5) mRNA levels (P < 0.05). AFB1, however, did not affect CYP450 3A37 mRNA levels. Supplementation with 2 μM SeMet protected against AFB1-induced changes and significantly increased selenoprotein W (SelW) mRNA levels (P < 0.05). Additionally, SelW knockdown attenuated the protective effect of SeMet on AFB1-induced damage and significantly increased the level of CYP450 1A5 expression (P < 0.05). Therefore, SeMet alleviates AFB1-induced damage in primary chicken hepatocytes by improving SelW expression, thus inhibiting CYP450 1A5 expression.
Author(s): Chen X, Che C, Korolchuk VI, Gan F, Pan C, Huang K
Publication type: Article
Publication status: Published
Journal: Journal of Agricultural and Food Chemistry
Print publication date: 29/03/2017
Online publication date: 13/03/2017
Acceptance date: 12/03/2017
ISSN (print): 0021-8561
ISSN (electronic): 1520-5118
Publisher: American Chemical Society
PubMed id: 28285519
Altmetrics provided by Altmetric