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DNA origami nanorobot fiber optic genosensor to TMV

Lookup NU author(s): Dr Emanuela Torelli

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This work is licensed under a Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International License (CC BY-NC-ND).


Abstract

In the quest of greater sensitivity and specificity of diagnostic systems, one continually searches for alternative DNA hybridization methods, enabling greater versatility and where possible field-enabled detection of target analytes. We present, herein, a hybrid molecular self-assembled scaffolded DNA origami entity, intimately immobilized via capture probes linked to aminopropyltriethoxysilane, onto a glass optical fiber end-face transducer, thus producing a novel biosensor. Immobilized DNA nanorobots with a switchable flap can then be actuated by a specific target DNA present in a sample, by exposing a hemin/G-quadruplex DNAzyme, which then catalyzes the generation of chemiluminescence, once the specific fiber probes are immersed in a luminol-based solution. Integrating organic nanorobots to inorganic fiber optics creates a hybrid system that we demonstrate as a proof-of-principle can be utilized in specific DNA sequence detection. This system has potential applications in a wide range of fields, including point-of-care diagnostics or cellular in vivo biosensing when using ultrathin fiber optic probes for research purposes.


Publication metadata

Author(s): Torelli E, Manzano M, Srivastava SK, Marks RS

Publication type: Article

Publication status: Published

Journal: Biosensors and Bioelectronics

Year: 2018

Volume: 99

Pages: 209-215

Print publication date: 15/01/2018

Online publication date: 21/07/2017

Acceptance date: 20/07/2017

Date deposited: 22/08/2017

ISSN (print): 0956-5663

ISSN (electronic): 1873-4235

Publisher: Elsevier

URL: https://doi.org/10.1016/j.bios.2017.07.051

DOI: 10.1016/j.bios.2017.07.051


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