Lookup NU author(s): Dr Jon Marles-Wright
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The site-specific recombinase Cre was previously reported to have in vitro activity. Here, we describe the method of purifying two new tyrosine site-specific recombinases VCre and Dre along with Cre by nickel affinity chromatography. We proved the in vitro function of the VCre and Dre on their respective conditional recombination sites. We also developed a methodology to one-step construct and optimize the productivity of a biosynthetic pathway through the combinatorial integration of promoters into a plasmid-encoded pathway by simply incubating a DNA mixture with recombinase system at 37 °C in vitro.
Author(s): Liu W, Tuck L, Marles-Wright J, Cai Y
Editor(s): Eroshenko N
Series Editor(s): John M. Walker
Publication type: Book Chapter
Publication status: Published
Book Title: Methods in Molecular Biology: Site Specific Recombinases
Online publication date: 17/08/2017
Acceptance date: 01/01/1900
Series Title: Methods in Molecular Biology
Publisher: Humana Press
Place Published: New York
PubMed id: 28815507
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