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Polyelectrolyte multi-layers assembly of SiCHA nanopowders and collagen type I on aminolysed PLA films to enhance cell-material interactions

Lookup NU author(s): Yanny Baba Ismail, Dr Ana Ferreira-Duarte, Dr Oana Bretcanu, Professor Kenneth Dalgarno

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This work is licensed under a Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International License (CC BY-NC-ND).


Abstract

This paper presents a new approach in assembling bone extracellular matrix components onto PLA films, and investigates the most favourable environment which can be created using the technique for cell-material interactions. Poly (lactic acid) (PLA) films were chemically modified by covalently binding the poly(ethylene imine) (PEI) as to prepare the substrate for immobilization of polyelectrolyte multilayers (PEMs) coating. Negatively charged polyelectrolyte consists of well-dispersed silicon-carbonated hydroxyapatite (SiCHA) nanopowders in hyaluronic acid (Hya) was deposited onto the modified PLA films followed by SiCHA in collagen type I as the positively charged polyelectrolyte. The outermost layer was finally cross-linked by 1-ethyl-3-(3-dimethylaminopropyl) carbodiimide hydrocholoride and N-hydroxysulfosuccinimide sodium salt (EDC/NHS) solutions. The physicochemical features of the coated PLA films were monitored via X-ray Photoelectron Spectroscopy (XPS) and Atomic Force Microscope (AFM). The amounts of calcium and collagen deposited on the surface were qualitatively and quantitatively determined. The surface characterizations suggested that 5-BL has the optimum surface roughness and highest amounts of calcium and collagen depositions among tested films. In vitro human mesenchymal stem cells (hMSCs) cultured on the coated PLA films confirmed that the coating materials greatly improved cell attachment and survival compared to unmodified PLA films. The cell viability, cell proliferation and Alkaline Phosphatase (ALP) expression on 5-BL were found to be the most favourable of the tested films. Hence, this newly developed coating materials assembly could contribute to the improvement of the bioactivity of polymeric materials and structures aimed to bone tissue engineering applications.


Publication metadata

Author(s): Baba Ismail YM, Ferreira AM, Bretcanu O, Dalgarno K, El Haj AJ

Publication type: Article

Publication status: Published

Journal: Colloids and Surfaces B: Biointerfaces

Year: 2017

Volume: 159

Pages: 445-453

Print publication date: 01/11/2017

Online publication date: 14/08/2017

Acceptance date: 25/07/2017

Date deposited: 17/04/2018

ISSN (print): 0927-7765

ISSN (electronic): 1873-4367

Publisher: Elsevier

URL: https://doi.org/10.1016/j.colsurfb.2017.07.086

DOI: 10.1016/j.colsurfb.2017.07.086


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