Lookup NU author(s): Dr Jon Sellars
Full text for this publication is not currently held within this repository. Alternative links are provided below where available.
© 2016 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim The identification and quantification of functional cytochromes P450 (CYPs) in biological samples is proving important for robust analyses of drug efficacy and metabolic disposition. In this study, a novel CYP activity-based probe was rationally designed and synthesised, demonstrating selective binding of CYP isoforms. The dependence of probe binding upon the presence of NADPH permits the selective detection of functionally active CYP. This allows the detection and analysis of these enzymes using biochemical and proteomic methodologies and approaches.
Author(s): Sellars JD, Skipsey M, Sadr-ul-Shaheed, Gravell S, Abumansour H, Kashtl G, Irfan J, Khot M, Pors K, Patterson LH, Sutton CW
Publication type: Article
Publication status: Published
Print publication date: 08/06/2016
Online publication date: 06/05/2016
Acceptance date: 04/03/2016
ISSN (print): 1860-7179
ISSN (electronic): 1860-7187
Publisher: John Wiley and Sons Ltd
PubMed id: 27154431
Altmetrics provided by Altmetric