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Lookup NU author(s): Dr Fernando Santos Beneit
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© 2017 Springer-Verlag GmbH Germany In this work, we identified glucose and glycerol as tacrolimus repressing carbon sources in the important species Streptomyces tsukubaensis. A genome-wide analysis of the transcriptomic response to glucose and glycerol additions was performed using microarray technology. The transcriptional time series obtained allowed us to compare the transcriptomic profiling of S. tsukubaensis growing under tacrolimus producing and non-producing conditions. The analysis revealed important and different metabolic changes after the additions and a lack of transcriptional activation of the fkb cluster. In addition, we detected important differences in the transcriptional response to glucose between S. tsukubaensis and the model species Streptomyces coelicolor. A number of genes encoding key players of morphological and biochemical differentiation were strongly and permanently downregulated by the carbon sources. Finally, we identified several genes showing transcriptional profiles highly correlated to that of the tacrolimus biosynthetic pathway regulator FkbN that might be potential candidates for the improvement of tacrolimus production.
Author(s): Ordonez-Robles M, Santos-Beneit F, Albillos SM, Liras P, Martin JF, Rodriguez-Garcia A
Publication type: Article
Publication status: Published
Journal: Applied Microbiology and Biotechnology
Year: 2017
Volume: 101
Issue: 22
Pages: 8181-8195
Print publication date: 01/11/2017
Online publication date: 05/10/2017
Acceptance date: 18/09/2017
ISSN (print): 0175-7598
ISSN (electronic): 1432-0614
Publisher: Springer Verlag
URL: https://doi.org/10.1007/s00253-017-8545-5
DOI: 10.1007/s00253-017-8545-5
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