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Integration of Single Cell Traps, Chemical Gradient Generator and Photosensors in a Microfluidic Platform for the Study of Alpha-Synuclein Toxicity in Yeast

Lookup NU author(s): Professor Tiago OuteiroORCiD

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This work is licensed under a Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International License (CC BY-NC-ND).

Abstract

© 2014 The Authors. Published by Elsevier Ltd. Alpha-synuclein (aSyn) is a key player in Parkinson's disease. Genetically engineered yeast cells producing aSyn fused with GFP (aSyn-GFP) have been used to study this protein. In this work, we present a microfluidic platform with integrated photosensors that captures single yeast cells in arrays of hydrodynamic traps and exposes them to a chemical gradient of precise composition. This platform enables the study of the effects of aSyn expression level and aggregation in genetically modified yeast cells by chemical stimulation. The photosensors allow the detection of cells in the traps by measuring the variations in light transmission or of the fluorescence produced by aSyn-GFP for real-time signal acquisition.

Publication metadata

Author(s): Fernandes JTS, Tenreiro S, Pedrosa CR, Gameiro A, Chu V, Outeiro TF, Conde JP

Publication type: Article

Publication status: Published

Journal: Procedia Engineering

Year: 2014

Volume: 87

Pages: 92-95

Online publication date: 31/12/2014

Acceptance date: 01/01/1900

Date deposited: 19/12/2017

ISSN (print): 1877-7058

Publisher: Elsevier Ltd

URL: https://doi.org/10.1016/j.proeng.2014.11.273

DOI: 10.1016/j.proeng.2014.11.273

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