Lookup NU author(s): Professor Tiago Outeiro
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Alpha-synuclein (aSyn) is implicated in Parkinson's disease and several other neurodegenerative disorders. To date, the function and intracellular dynamics of aSyn are still unclear. Here, we tracked the dynamics of aSyn using photoactivatable green fluorescent protein as a reporter. We found that the availability of the aSyn N terminus modulates its shuttling into the nucleus. Interestingly, familial aSyn mutations altered the dynamics at which the protein distributes throughout the cell. Both the A30P and A53T aSyn mutations increase the speed at which the protein moves between the nucleus and cytoplasm, respectively. We also found that specific kinases potentiate the shuttling of aSyn between nucleus and cytoplasm. A mutant aSyn form that blocks S129 phos-phorylation, S129A, results in the formation of cytoplasmic inclusions, suggesting phosphorylation modulates aggregation in addition to modulating aSyn intracellular dynamics. Finally, we found that the molecular chaperone HSP70 accelerates the entry of aSyn into the nuclear compartment. © The Author(s) 2013.
Author(s): Goncalves S, Outeiro TF
Publication type: Article
Publication status: Published
Journal: Molecular Neurobiology
Print publication date: 01/06/2013
Online publication date: 08/02/2013
ISSN (print): 0893-7648
ISSN (electronic): 1559-1182
PubMed id: 23389286
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