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Enzymatic fuel cells with an oxygen resistant variant of pyranose-2-oxidase as anode biocatalyst

Lookup NU author(s): Samet Şahin, Dr Eileen Yu

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This work is licensed under a Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International License (CC BY-NC-ND).


Abstract

© 2018 In enzymatic fuel cells (EnFCs), hydrogen peroxide formation is one of the main problems when enzymes, such as, glucose oxidase (GOx) is used due to the conversion of oxygen to hydrogen peroxide in the catalytic reaction. To address this problem, we here report the first demonstration of an EnFC using a variant of pyranose-2-oxidase (P2O-T169G) which has been shown to have low activity towards oxygen. A simple and biocompatible immobilisation approach incorporating multi-walled-carbon nanotubes within ferrocene (Fc)-Nafion film was implemented to construct EnFCs. Successful immobilisation of the enzymes was demonstrated showing 3.2 and 1.7-fold higher current than when P2O-T169G and GOx were used in solution, respectively. P2O-T169G showed 25% higher power output (maximum power density value of 8.45 ± 1.6 μW cm−2) and better stability than GOx in aerated glucose solutions. P2O-T169G maintained > 70% of its initial current whereas GOx lost activity > 90% during the first hour of 12 h operation at 0.15 V (vs Ag/Ag+). A different fuel cell configuration using gas-diffusion cathode and carbon paper electrodes were used to improve the power output of the fuel cell to 29.8 ± 6.1 µW cm−2. This study suggests that P2O-T169G with low oxygen activity could be a promising anode biocatalyst for EnFC applications.


Publication metadata

Author(s): Sahin S, Wongnate T, Chuaboon L, Chaiyen P, Yu EH

Publication type: Article

Publication status: Published

Journal: Biosensors and Bioelectronics

Year: 2018

Volume: 107

Pages: 17-25

Print publication date: 01/06/2018

Online publication date: 01/02/2018

Acceptance date: 30/01/2018

ISSN (print): 0956-5663

ISSN (electronic): 1873-4235

Publisher: Elsevier Ltd

URL: https://doi.org/10.1016/j.bios.2018.01.065

DOI: 10.1016/j.bios.2018.01.065

Data Source Location: http://dx.doi.org/10.17634/154300-78


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