Toggle Main Menu Toggle Search

Open Access padlockePrints

An In Vitro Human Skin Test for Assessing Adverse Immune Reactions and Sensitization Potential

Lookup NU author(s): Professor Anne Dickinson, Dr Xiao Wang, Dr Shaheda Ahmed

Downloads

Full text for this publication is not currently held within this repository. Alternative links are provided below where available.


Abstract

© Springer International Publishing AG 2017. A human in vitro skin explant test was developed by Alcyomics for the prediction of adverse immune reactions and assessment of relative potency. The skin explant test has a unique readout of histological damage, which ranges in severity from grade 0 and I (negative reaction) to grades II, III and IV damage (positive reaction) in human full-thickness skin. The test has been assessed for predicting responses to sensitizers and non-sensitizers, originally tested in the mouse local lymph node assay (LLNA). Results compared with the LLNA gave 95% specificity, 95% sensitivity and 95% concordance with a correlation coefficient of 0.9. Similar specificity and sensitivity were achieved for comparison of results to published human data with a correlation coefficient of 0.91. The test can also identify chemicals mis-classified in the mouse local lymph node assay (LLNA). Results of the skin test correlate with high or low T cell proliferation and IFNγ production, and these assays can be used as in vitro screening tests for compounds. Collectively, the human in vitro skin explant test can provide a robust, reliable and novel approach for characterization of sensitizing activity as a first step in the risk assessment process.


Publication metadata

Author(s): Dickinson A, Nong Wang X, Ahmed S

Publication type: Book Chapter

Publication status: Published

Book Title: Alternatives for Dermal Toxicity Testing

Year: 2017

Pages: 437-448

Online publication date: 19/06/2017

Acceptance date: 02/04/2016

Publisher: Springer International Publishing

URL: https://doi.org/10.1007/978-3-319-50353-0_30

DOI: 10.1007/978-3-319-50353-0_30

Library holdings: Search Newcastle University Library for this item

ISBN: 9783319503530


Actions

Link to this publication


Share