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Lookup NU author(s): Professor David Lydall
This work is licensed under a Creative Commons Attribution 4.0 International License (CC BY 4.0).
Saccharomyces cerevisiae is a commonly used model organism for understanding eukaryotic gene function. However, the close proximity between yeast genes can complicate the interpretation of yeast genetic data, particularly high-throughput data. In this study, we examined the interplay between genes encoding components of the PAF1 complex and VPS36, the gene located next to CDC73 on chromosome XII. The PAF1 complex (Cdc73, Paf1, Ctr9, Leo1, and Rtf1, in yeast) affects RNA levels by affecting transcription, histone modifications, and post-transcriptional RNA processing. The human PAF1 complex is linked to cancer, and in yeast, it has been reported to play a role in telomere biology. Vps36, part of the ESCRT-II complex, is involved in sorting proteins for vacuolar/lysosomal degradation. We document a complex set of genetic interactions, which include an adjacent gene effect between CDC73 and VPS36 and synthetic sickness between vps36? and cdc73?, paf1?, or ctr9?. Importantly, paf1? and ctr9? are synthetically lethal with deletions of other components of the ESCRT-II (SNF8 and VPS25), ESCRT-I (STP22), or ESCRT-III (SNF7) complexes. We found that RNA levels of VPS36, but not other ESCRT components, are positively regulated by all components of the PAF1 complex. Finally, we show that deletion of ESCRT components decreases the telomere length in the S288C yeast genetic background, but not in the W303 background. Together, our results outline complex interactions, in cis and in trans, between genes encoding PAF1 and ESCRT-II complex components that affect telomere function and cell viability in yeast.
Author(s): Rodrigues J, Lydall D
Publication type: Article
Publication status: Published
Journal: Current Genetics
Year: 2018
Volume: 64
Issue: 5
Pages: 1105-1116
Print publication date: 01/10/2018
Online publication date: 22/03/2018
Acceptance date: 17/03/2018
Date deposited: 23/03/2018
ISSN (print): 0172-8083
ISSN (electronic): 1432-0983
Publisher: Springer
URL: https://doi.org/10.1007/s00294-018-0828-6
DOI: 10.1007/s00294-018-0828-6
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