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Rapid detection of Fusarium oxysporum f. sp. lactucae on soil, lettuce seeds and plants using loop-mediated isothermal amplification

Lookup NU author(s): Professor Neil Boonham

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This is the authors' accepted manuscript of an article that has been published in its final definitive form by Wiley-Blackwell, 2018.

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Abstract

© 2018 British Society for Plant Pathology. Fusarium oxysporum f. sp. lactucae (FOL) is a soil- and seedborne pathogen and the causal agent of fusarium wilt on lettuce. Four races have been identified within FOL, with different worldwide distribution. Several molecular techniques have been used to detect and identify this pathogen; however, not all of them have the optimal characteristics in terms of sensitivity to perform FOL detection in plant and seed material. A loop-mediated isothermal amplification (LAMP) assay was developed based on the sequence-characterized amplified region (SCAR) obtained in a previous rapid amplification of polymorphic DNA (RAPD) study. The LAMP assay has been validated according to the EPPO standard PM7/98. The LAMP assay was tested with lettuce seeds, soil and plant material, and can be used successfully to amplify DNA from each of these matrices. In seed lots artificially inoculated with FOL, the detection limit of the LAMP test was 0.004% infected seed. Plant Pathology


Publication metadata

Author(s): Franco Ortega S, Tomlinson J, Gilardi G, Spadaro D, Gullino ML, Garibaldi A, Boonham N

Publication type: Article

Publication status: Published

Journal: Plant Pathology

Year: 2018

Volume: 67

Issue: 7

Pages: 1462-1473

Print publication date: 01/09/2018

Online publication date: 14/03/2018

Acceptance date: 02/04/2016

ISSN (print): 0032-0862

ISSN (electronic): 1365-3059

Publisher: Wiley-Blackwell

URL: https://doi.org/10.1111/ppa.12855

DOI: 10.1111/ppa.12855


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