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Bloch surface wave label-free and fluorescence platform for the detection of VEGF biomarker in biological matrices

Lookup NU author(s): Dr Subinoy Rana

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Abstract

© 2017 Elsevier B.V. We report on the detection of an angiogenic molecule Vascular Endothelial Growth Factor (VEGF) in different biological matrices by means of a new integrated biosensing platform exploiting the properties of Bloch surface waves. The new platform takes advantage of a tandem configuration, in which both label-free and enhanced fluorescence detection are implemented. Specifically designed one dimensional photonic crystals were deposited directly on disposable and low cost plastic biochips. A direct sandwich immunoassay was used to detect VEGF in buffer, cell culture supernatant and human plasma at low concentration (ng/mL). The platform enabled the detection of VEGF in all three matrices with high resolution, fast turnaround time (30 min) and in close agreement with the results of enzyme linked immunosorbent assays.


Publication metadata

Author(s): Rizzo R, Alvaro M, Danz N, Napione L, Descrovi E, Schmieder S, Sinibaldi A, Chandrawati R, Rana S, Munzert P, Schubert T, Maillart E, Anopchenko A, Rivolo P, Mascioletti A, Sonntag F, Stevens MM, Bussolino F, Michelotti F

Publication type: Article

Publication status: Published

Journal: Sensors and Actuators, B: Chemical

Year: 2018

Volume: 255

Pages: 2143-2150

Print publication date: 01/02/2018

Online publication date: 06/09/2017

Acceptance date: 04/09/2017

Publisher: Elsevier B.V.

URL: https://doi.org/10.1016/j.snb.2017.09.018

DOI: 10.1016/j.snb.2017.09.018


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