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Lookup NU author(s): Dr Subinoy Rana
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© 2017 Elsevier B.V. We report on the detection of an angiogenic molecule Vascular Endothelial Growth Factor (VEGF) in different biological matrices by means of a new integrated biosensing platform exploiting the properties of Bloch surface waves. The new platform takes advantage of a tandem configuration, in which both label-free and enhanced fluorescence detection are implemented. Specifically designed one dimensional photonic crystals were deposited directly on disposable and low cost plastic biochips. A direct sandwich immunoassay was used to detect VEGF in buffer, cell culture supernatant and human plasma at low concentration (ng/mL). The platform enabled the detection of VEGF in all three matrices with high resolution, fast turnaround time (30 min) and in close agreement with the results of enzyme linked immunosorbent assays.
Author(s): Rizzo R, Alvaro M, Danz N, Napione L, Descrovi E, Schmieder S, Sinibaldi A, Chandrawati R, Rana S, Munzert P, Schubert T, Maillart E, Anopchenko A, Rivolo P, Mascioletti A, Sonntag F, Stevens MM, Bussolino F, Michelotti F
Publication type: Article
Publication status: Published
Journal: Sensors and Actuators, B: Chemical
Year: 2018
Volume: 255
Pages: 2143-2150
Print publication date: 01/02/2018
Online publication date: 06/09/2017
Acceptance date: 04/09/2017
Publisher: Elsevier B.V.
URL: https://doi.org/10.1016/j.snb.2017.09.018
DOI: 10.1016/j.snb.2017.09.018
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