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Structure and function of a novel periplasmic chitooligosaccharide-binding protein from marine Vibrio bacteria

Lookup NU author(s): Dr Wipa Suginta, Dr David Bulmer, Professor Bert van den Berg

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Abstract

© 2018 by The American Society for Biochemistry and Molecular Biology, Inc. Periplasmic solute-binding proteins in bacteria are involved in the active transport of nutrients into the cytoplasm. In marine bacteria of the genus Vibrio, a chitooligosaccharide-binding protein (CBP) is thought to be the major solute-binding protein controlling the rate of chitin uptake in these bacteria. However, the molecular mechanism of the CBP involvement in chitin metabolism has not been elucidated. Here, we report the structure and function of a recombinant chitooligosaccharide-binding protein from Vibrio harveyi, namely VhCBP, expressed in Escherichia coli. Isothermal titration calorimetry revealed that VhCBP strongly binds shorter chitooligosaccharides ((GlcNAc)n, where n 2, 3, and 4) with affinities that are considerably greater than those for glycoside hydrolase family 18 and 19 chitinases but does not bind longer ones, including insoluble chitin polysaccharides. We also found that VhCBP comprises two domains with flexible linkers and that the domain– domain interface forms the sugar-binding cleft, which is not long extended but forms a small cavity. (GlcNAc)2 bound to this cavity, apparently triggering a closed conformation of VhCBP. Trp-363 and Trp-513, which stack against the two individual GlcNAc rings, likely make a major contribution to the high affinity of VhCBP for (GlcNAc)2. The strong chitobiose binding, followed by the conformational change of VhCBP, may facilitate its interaction with an active-transport system in the inner membrane of Vibrio species.


Publication metadata

Author(s): Suginta W, Sritho N, Ranok A, Bulmer DM, Kitaoku Y, van den Berg B, Fukamizo T

Publication type: Article

Publication status: Published

Journal: Journal of Biological Chemistry

Year: 2018

Volume: 293

Issue: 14

Pages: 5150-5159

Print publication date: 06/04/2018

Online publication date: 14/02/2018

Acceptance date: 11/02/2018

ISSN (print): 0021-9258

ISSN (electronic): 1083-351X

Publisher: American Society for Biochemistry and Molecular Biology Inc.

URL: https://doi.org/10.1074/jbc.RA117.001012

DOI: 10.1074/jbc.RA117.001012


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