Lookup NU author(s): Claire Whitworth,
Dr Chris Redfern,
Dr Timothy Cheek
This work is licensed under a Creative Commons Attribution 4.0 International License (CC BY 4.0).
Neurogenesis is a complex process leading to the generation of neuronal networks and glial cell types from stem cells or intermediate progenitors. Mapping subcellular and molecular changes accompanying the switch from proliferation to differentiation is vital for developing therapeutic targets for neurological diseases. Neuronal (N-type) and glial (S-type) phenotypes within the SH-SY5Y neuroblastoma cell line have distinct differentiation responses to 9-cis-retinoic acid (9cRA). In both cell phenotypes, these were accompanied at the single cell level by an uncoupling of Ca2+ store release from store-operated Ca2+ entry (SOCE), mediated by changes in the expression of calcium release-activated calcium pore proteins. This remodelling of calcium signalling was moderated by the predominant cell phenotype within the population. N- and S-type cells differed markedly in their phenotypic stability after withdrawal of the differentiation inducer, with the phenotypic stability of S-type cells, both morphologically and with respect to SOCE properties, in marked contrast to the lability of the N-type phenotype. Furthermore, the SOCE response of I-type cells, a presumed precursor to both N- and S-type cells, varied markedly in different cell environments. These results demonstrate the unique biology of neuronal and glial derivatives of common precursors and suggest that direct or indirect interactions between cell types are vital components of neurogenesis that need to be considered in experimental models.
Author(s): Whitworth CL, Redfern CPF, Cheek TR
Publication type: Article
Publication status: Published
Journal: Molecular Neurobiology
Print publication date: 01/02/2019
Online publication date: 26/05/2018
Acceptance date: 09/05/2018
ISSN (print): 0893-7648
ISSN (electronic): 1559-1182
PubMed id: 29802571
Altmetrics provided by Altmetric