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Nonomuraea insulae sp. nov., isolated from forest soil

Lookup NU author(s): Dr Imen Nouioui, Professor Hans-Peter Klenk

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This is the authors' accepted manuscript of an article that has been published in its final definitive form by Springer Netherlands, 2018.

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Abstract

© 2018 Springer International Publishing AG, part of Springer Nature Strain H2R21T, a novel actinobacterium, isolated from a forest soil sample collected from Heybeliada, Istanbul, Turkey, and a polyphasic approach was used for characterisation of the strain. Chemotaxonomic and morphological characterisation of strain H2R21T indicated that it belongs to the genus Nonomuraea. 16S rRNA gene sequence similarity showed that the strain is closely related to Nonomuraea purpurea 1SM4-01T (99.1%) and Nonomuraea solani CGMCC 4.7037T (98.4%). DNA–DNA relatedness values were found to be lower than 70% between the isolate and its phylogenetic neighbours N. purpurea 1SM4-01T, N. solani CGMCC 4.7037T and Nonomuraea rhizophila YIM 67092T. The whole cell hydrolysates of strain H2R21T were found to contain meso-diaminopimelic acid as the diagnostic diamino acid and glucose, madurose, mannose and ribose as the cell sugars. The polar lipids were identified as phosphatidylglycerol, diphosphatidylglycerol, phosphatidylmethylethanolamine, phosphatidylethanolamine, hydroxy-phosphatidylethanolamine, dihydroxy-phosphatidylethanolamine, phosphatidylinositol, glycophosphatidylinositol, two glycophospholipids and two unidentified lipids. The predominant menaquinones were identified as MK-9(H4) and MK-9(H6). The major fatty acids were found to be iso-C16:0, iso-C16:0 2OH and C17:0 10-methyl. On the basis of DNA–DNA relatedness data and some phenotypic characteristics, it is evident that strain H2R21T can be distinguished from the closely related species in the genus Nonomuraea. Thus, it is concluded that strain H2R21T represents a novel species of the genus Nonomuraea, for which the name Nonomuraea insulae sp. nov. is proposed. The type strain is H2R21T (= DSM 102915T = CGMCC 4.7338T = KCTC 39769T).


Publication metadata

Author(s): Saricaoglu S, Nouioui I, Ay H, Saygin H, Bektas KI, Guven K, Cetin D, Klenk H-P, Isik K, Sahin N

Publication type: Article

Publication status: Published

Journal: Antonie van Leeuwenhoek

Year: 2018

Volume: 111

Issue: 11

Pages: 2051-2059

Print publication date: 01/11/2018

Online publication date: 16/05/2018

Acceptance date: 11/05/2018

Date deposited: 12/07/2018

ISSN (print): 0003-6072

ISSN (electronic): 1572-9699

Publisher: Springer Netherlands

URL: https://doi.org/10.1007/s10482-018-1097-6

DOI: 10.1007/s10482-018-1097-6


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