Lookup NU author(s): Professor Nicolas Tsesmetzis
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© 2018 The Author(s) Significant low-cost biofuel production volumes could be achieved from commercial-scale silage by redirecting lactic acid fermentation to ethanol production. A temporal metagenomic analysis on ensiled sweet sorghum inoculated with an ethanologenic yeast has been conducted to understand the underlying microbial processes during bioethanol production. Individual silage buckets approximating silage piles were prepared with freshly harvested material and supplemented with ethanologenic yeast, sulfuric acid or both. The ensiling progress was assessed using high performance liquid chromatography, microbial taxonomic identification and abundance. The combined treatment with Saccharomyces and acid led to a steady reduction of bacterial abundance and microbial diversity with Lactobacillus becoming the dominant genus during the late timepoints. Furthermore, the addition of acid to inhibit bacterial growth hindered Saccharomyces ability to compete with native yeasts like Candida. Knowledge of the response of the in-situ microbial community to the various treatments during ensiling will help improve current methodologies for bioethanol production.
Author(s): Gallagher D, Parker D, Allen DJ, Tsesmetzis N
Publication type: Article
Publication status: Published
Journal: Bioresource Technology
Print publication date: 01/09/2018
Online publication date: 26/05/2018
Acceptance date: 12/05/2018
ISSN (print): 0960-8524
ISSN (electronic): 1873-2976
Publisher: Elsevier Ltd
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